Rapiddecalcificationoftemporalboneswithpreservationofultrastructure
victoria j. maddena and miriam m. hensonb, *
a microscopy services laboratory, department of pathology and laboratory medicine, the university of north carolina, chapel hill, nc 27599, usa
b division of otolaryngology/head and neck surgery, department of surgery, cb #7090, taylor hall, the university of north carolina, chapel hill, nc 27599, usa
received 12 march 1997;
revised 13 june 1997;
accepted 17 june 1997.
available online 20 may 1998.
abstractdecalcification of temporal bones, especially from primates, has routinely required long periods of time and has been a major deterrent to many types of morphological studies. in this investigation, temporal bones from the monkey, macaca fuscata, were decalcified with ethylene diamine tetraacetic acid (edta) in a microwave oven. to isolate effects of microwaves on decalcification, tissue was fixed and embedded using routine methods; only decalcification was carried out in the microwave oven. the procedure is described in detail. instead of months, decalcification was complete in two working days. control procedures included decalcification at room temperature and use of a regular oven at a temperature equal to that reached in the microwave. the ultrastructure of cochlear tissue was equal to or better than that obtained with routine decalcification.
author keywords: temporal bone; inner ear; primate; microwave; decalcification; electron microscopy
*corresponding author. .: +1 (919) 966-3801; : +1 (919) 966-1856; : mmhenson@med.unc.edu
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Rapiddecalcificationoftemporalboneswithpreservationofultrastructure
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礦用雙抗涂塑螺旋鋼管有什么用途
月餅成型機加工效率與口味的雙重保障
檢測紙和紙板吸水性能的設(shè)備（可勃法）
一級承試資質(zhì)設(shè)備的作用都有什么？
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a microscopy services laboratory, department of pathology and laboratory medicine, the university of north carolina, chapel hill, nc 27599, usa
b division of otolaryngology/head and neck surgery, department of surgery, cb #7090, taylor hall, the university of north carolina, chapel hill, nc 27599, usa
received 12 march 1997;
revised 13 june 1997;
accepted 17 june 1997.
available online 20 may 1998.
abstractdecalcification of temporal bones, especially from primates, has routinely required long periods of time and has been a major deterrent to many types of morphological studies. in this investigation, temporal bones from the monkey, macaca fuscata, were decalcified with ethylene diamine tetraacetic acid (edta) in a microwave oven. to isolate effects of microwaves on decalcification, tissue was fixed and embedded using routine methods; only decalcification was carried out in the microwave oven. the procedure is described in detail. instead of months, decalcification was complete in two working days. control procedures included decalcification at room temperature and use of a regular oven at a temperature equal to that reached in the microwave. the ultrastructure of cochlear tissue was equal to or better than that obtained with routine decalcification.
author keywords: temporal bone; inner ear; primate; microwave; decalcification; electron microscopy
*corresponding author. .: +1 (919) 966-3801; : +1 (919) 966-1856; : mmhenson@med.unc.edu
焦化行業(yè)有效推進超低排放改造的措施
天拓四方受邀參加2018西門子用戶大會并分享MOM行業(yè)解決方案
救護車洗消站建設(shè)規(guī)范方案
食品機械分析
菲尼克斯 DC/DC轉(zhuǎn)換器 2320092 初級開關(guān)模式
Rapiddecalcificationoftemporalboneswithpreservationofultrastructure
IL PN BK DI8 DO4 2SCRJ-PAC耦合器詳細文章
怎么判斷焊接效果的好壞
二手耙式干燥機
土壤熱解吸法可以有效的去除土壤中的有機氯農(nóng)藥
電動調(diào)節(jié)型三片式球閥Q911F電動調(diào)節(jié)型不銹鋼內(nèi)螺紋球閥的特點
探索科技未知：微納3D打印技術(shù)賦能軟體機器人
過瘤胃胍基乙酸廠家
土壤鹽度電導(dǎo)率測量儀2266FS的工作原理及使用
礦用雙抗涂塑螺旋鋼管有什么用途
月餅成型機加工效率與口味的雙重保障
檢測紙和紙板吸水性能的設(shè)備（可勃法）
一級承試資質(zhì)設(shè)備的作用都有什么？
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